rat hepatoma cell line  (ATCC)

Journal: Hepatic Oncology

Article Title: APT imaging of hepatocellular carcinoma signals an effective therapeutic response in advance of tumor shrinkage

doi: 10.1080/20450923.2024.2389031

Figure Lengend Snippet: Changes in APT signal intensity (as a percentage) for the N1S1 tumors following in LDL-OA nanoparticle treatment. (Upper panel) T 2 -weighted axial MRI (lower panel) APT imaged co-registered with T2 axial MRI. The ATP image masks are overlaid the liver and tumor region. (Insert upper) Change in the APT signal in liver and tumor tissue before, 24 and 72 h post LDL-OA treatment. (Insert lower) H&E stained section showing liver and tumor interface. White arrows indicate tumor. Scale bar = 100 um.

Article Snippet: N1S1 rat hepatoma cell line (ATCC, CRL-1603, VA, USA) and the human liver tumor cell line HepG2 were cultured in Dulbecco's Modified Eagle's Medium (Sigma, D6429) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.

Techniques: Staining

Journal: Hepatic Oncology

Article Title: APT imaging of hepatocellular carcinoma signals an effective therapeutic response in advance of tumor shrinkage

doi: 10.1080/20450923.2024.2389031

Figure Lengend Snippet: Changes in APT signal intensity (as a percentage) for the N1S1 tumors following in LDL-DHA nanoparticle treatment. (Upper panel) T 2 -weighted axial MRI (lower panel) APT imaged co-registered with T2 axial MRI. The ATP image masks are overlaid the liver and tumor region. (Insert upper) Change in the APT signal in liver and tumor tissue before, 24 and 72 h post LDL-DHA treatment. (Insert lower) H&E stained section showing liver and tumor interface. White arrows indicate tumor. *** represents p < 0.001 difference between groups. Scale bar = 100 um.

Article Snippet: N1S1 rat hepatoma cell line (ATCC, CRL-1603, VA, USA) and the human liver tumor cell line HepG2 were cultured in Dulbecco's Modified Eagle's Medium (Sigma, D6429) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin.

Techniques: Staining

Journal: Liver Cancer

Article Title: Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model

doi: 10.1159/000502774

Figure Lengend Snippet: Gene expression and serum concentration of TGF-β1 in tumors. a Quantification of TGF-β1, α-SMA, HIF-1α, and collagen type-1α-1 chain mRNAs normalized to β-actin in tumors of the HAE group (black bars, n = 5) and the sham group (gray bars, n = 4) by qPCR. Asterisks indicate a higher (* p < 0.01) mRNA expression in tumors of the HAE group compared with the sham group according to Student t test. b Serum TGF-β1 protein of N1S1-bearing rats (HAE: n = 5; sham: n = 4). TGF-β1 secretion was not significantly different between the HAE and sham groups (p = 0.93) according to Student t test. HAE, hepatic arterial embolization; TGF-β1, transforming growth factor β1.

Article Snippet: Tumor Cell Line The N1S1 rat hepatoma cell lines were obtained from the manufacture (ATCC, Manassas, VA, USA) and used to in vivo and in vitro in this study.

Techniques: Gene Expression, Concentration Assay, Expressing

Journal: Liver Cancer

Article Title: Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model

doi: 10.1159/000502774

Figure Lengend Snippet: Hypoxia-induced expression of TGF-β1 and HIF-1α. a TGF-β1 and HIF-1α protein expression in rat hepatoma cells (N1S1) cultured under normoxic conditions (21% O2) with or without CoCl2 (100 μmol/L; white bar) for 24 h and under hypoxic conditions (1% O2; black bar) for 24 and 48 h. Representative western blots for TGF-β1 and HIF-1α protein expression (upper panel) and densitometric analysis results normalized to β-actin (lower panel) are shown. The protein expression of TGF-β1 and HIF-1α was higher (* p < 0.01) under hypoxic conditions than under normoxic conditions according to Student t test. b TGF-β1 secreted into the supernatant of N1S1 cells cultured under hypoxic conditions for 48 h was detected by ELISA. TGF-β1 secretion was higher (* p < 0.01) under hypoxic conditions than under normoxic conditions according to Student t test. Each experiment was performed 4 times (a, b). TGF-β1, transforming growth factor β1; HIF-1α, hypoxia-inducible factor 1α.

Article Snippet: Tumor Cell Line The N1S1 rat hepatoma cell lines were obtained from the manufacture (ATCC, Manassas, VA, USA) and used to in vivo and in vitro in this study.

Techniques: Expressing, Cell Culture, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Liver Cancer

Article Title: Hepatic Artery Embolization Induces the Local Overexpression of Transforming Growth Factor β1 in a Rat Hepatoma Model

doi: 10.1159/000502774

Figure Lengend Snippet: Relationship between TGF-β1 and HIF-1α expression. Effect of HIF-1α inhibitors on the hypoxia-induced expression of TGF-β1. a TGF-β1 secreted into the supernatant of N1S1 cells cultured under hypoxic conditions (1% O2) for 48 h without or with HIF-1α inhibitors (20 μmol/L LW6 or 300 nmol/L FM19G11) was detected by ELISA. The secretion of TGF-β1 under hypoxic conditions was significantly suppressed by HIF-1α inhibitors (* p < 0.01). b A representative western blot of HIF-1α expression in N1S1 cells stimulated with TGF-β1 (5 ng/mL) under normoxic conditions for 48 h (upper panel) and densitometric analysis results normalized to β-actin (lower panel). TGF-β1 significantly induced HIF-1α protein expression under normoxic conditions (* p < 0.01). Each experiment was performed 4 times (a, b). TGF-β1, transforming growth factor; HIF-1α, hypoxia-inducible factor 1α.

Article Snippet: Tumor Cell Line The N1S1 rat hepatoma cell lines were obtained from the manufacture (ATCC, Manassas, VA, USA) and used to in vivo and in vitro in this study.

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot